8VTE

Co-structure of the Fab of the anti-TIGIT Vibostolimab antibody with its antigen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	300
Detector technology	PIXEL
Collection date	2020-09-19
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	213.689, 213.689, 66.428
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.210 - 2.290
R-factor	0.2305
Rwork	0.229
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.990
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.210	2.502
High resolution limit [Å]	2.290	2.290
Rmerge	0.091
Number of reflections	49814	2493
<I/σ(I)>	14.6	1.6
Completeness [%]	93.7
Redundancy	6.7
CC(1/2)	0.999	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	300	0.02M D-Glucose; 0.02M D-Mannose; 0.02M D-Galactose; 0.02M L-Fucose; 0.02M D-Xylose; 0.02M N-Acetyl-D-Glucosamine; 0.1M Imidazole, 0.1M MES; 20%v/v Glycerol; 10% PEG4K