8VTD
Co-structure of the Fab of the anti-TIGIT Vibostolimab antibody with its antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.775, 105.996, 71.758 |
| Unit cell angles | 90.00, 104.40, 90.00 |
Refinement procedure
| Resolution | 42.140 - 1.230 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS (20180409) |
| Data scaling software | Aimless (0.7.2) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.144 | 1.363 |
| High resolution limit [Å] | 1.230 | 1.231 |
| Rmerge | 0.045 | 0.652 |
| Rmeas | 0.053 | 0.775 |
| Rpim | 0.029 | 0.414 |
| Total number of observations | 20248 | |
| Number of reflections | 117862 | 5892 |
| <I/σ(I)> | 12.3 | 1.5 |
| Completeness [%] | 67.7 | |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 16% w/v PEG 20K |
