8VRS
Mucin 16 peptide fused to MBP in complex with 4H11-scFv antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 183 |
| Detector technology | PIXEL |
| Collection date | 2020-08-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.810, 112.634, 110.808 |
| Unit cell angles | 90.00, 101.25, 90.00 |
Refinement procedure
| Resolution | 56.430 - 2.470 |
| R-factor | 0.1936 |
| Rwork | 0.192 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.385 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.630 | 2.540 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.083 | 0.666 |
| Rmeas | 0.115 | 0.910 |
| Rpim | 0.079 | 0.617 |
| Number of reflections | 51394 | 4270 |
| <I/σ(I)> | 9.2 | 1.4 |
| Completeness [%] | 98.9 | 95.2 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.995 | 0.683 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.1M Sodium Citrate, pH 5.0, 10mM Barium Chloride, 27% PEG MME 5000 |
