8VRS
Mucin 16 peptide fused to MBP in complex with 4H11-scFv antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 183 |
Detector technology | PIXEL |
Collection date | 2020-08-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.810, 112.634, 110.808 |
Unit cell angles | 90.00, 101.25, 90.00 |
Refinement procedure
Resolution | 56.430 - 2.470 |
R-factor | 0.1936 |
Rwork | 0.192 |
R-free | 0.23690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.385 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 112.630 | 2.540 |
High resolution limit [Å] | 2.470 | 2.470 |
Rmerge | 0.083 | 0.666 |
Rmeas | 0.115 | 0.910 |
Rpim | 0.079 | 0.617 |
Number of reflections | 51394 | 4270 |
<I/σ(I)> | 9.2 | 1.4 |
Completeness [%] | 98.9 | 95.2 |
Redundancy | 3.5 | 3.4 |
CC(1/2) | 0.995 | 0.683 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.1M Sodium Citrate, pH 5.0, 10mM Barium Chloride, 27% PEG MME 5000 |