8VRR
4H11-scFv antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 183 |
| Detector technology | PIXEL |
| Collection date | 2020-03-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 51.592, 112.795, 150.668 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.330 - 2.360 |
| R-factor | 0.2278 |
| Rwork | 0.225 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.123 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.300 | 2.450 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.070 | 1.688 |
| Rmeas | 0.083 | 2.021 |
| Rpim | 0.045 | 1.098 |
| Number of reflections | 18408 | 1817 |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 99.3 | 94.3 |
| Redundancy | 6.5 | 6.2 |
| CC(1/2) | 0.999 | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.1M Sodium Citrate, pH 5.0, 20% PEG 4000 |
