8VQ8
Immune receptor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | H 3 |
| Unit cell lengths | 197.604, 197.604, 79.331 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.400 - 2.010 |
| R-factor | 0.1881 |
| Rwork | 0.187 |
| R-free | 0.21510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.836 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.050 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.116 | 1.296 |
| Rmeas | 0.124 | |
| Rpim | 0.043 | 0.454 |
| Number of reflections | 76994 | 4527 |
| <I/σ(I)> | 11 | 1.5 |
| Completeness [%] | 99.9 | 98.5 |
| Redundancy | 8.1 | 8 |
| CC(1/2) | 0.995 | 0.609 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293.15 | 0.1 M SPG pH 8.2, 5.0 %w/v PEG 1500 |
