8VFF

Binary DNA Polymerase Beta bound to DNA containing primer terminal FapydG base-paired with a dA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-15
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	54.399, 79.277, 54.885
Unit cell angles	90.00, 105.53, 90.00

Refinement procedure

Resolution	23.640 - 1.690
R-factor	0.2156
Rwork	0.215
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.251
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.730
High resolution limit [Å]	1.690	1.690
Number of reflections	43590	2102
<I/σ(I)>	17.5
Completeness [%]	93.4
Redundancy	1.8
CC(1/2)	0.998	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate