8VFA

Ternary DNA Polymerase Beta bound to DNA containing primer terminal dC base-paired with FapydG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-29
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	54.437, 79.531, 54.889
Unit cell angles	90.00, 108.26, 90.00

Refinement procedure

Resolution	24.940 - 2.050
R-factor	0.2286
Rwork	0.226
R-free	0.26670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.423
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.090
High resolution limit [Å]	2.050	2.050
Number of reflections	38424	1274
<I/σ(I)>	24.7
Completeness [%]	99.1
Redundancy	4.6
CC(1/2)	0.999	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate