8V5T
Crystal structure of Alzheimers disease phospholipase D3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 205.137, 60.566, 111.501 |
| Unit cell angles | 90.00, 98.72, 90.00 |
Refinement procedure
| Resolution | 48.960 - 2.300 |
| R-factor | 0.1865 |
| Rwork | 0.185 |
| R-free | 0.20940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.147 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.960 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.042 | 0.458 |
| Number of reflections | 112535 | 712 |
| <I/σ(I)> | 10.2 | 1.6 |
| Completeness [%] | 99.0 | 96.9 |
| Redundancy | 3.4 | 3.3 |
| CC(1/2) | 0.997 | 0.683 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.17 M ammonium sulfate 15% (v/v) glycerol 25% (w/v) PEG 4000 |
