8V5L
Structure of the Varicella Zoster Virus (VZV) gI binding domain of glycoprotein E (gE) in complex with human Fab 1A2 and 1E12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 218.776, 63.493, 96.484 |
| Unit cell angles | 90.00, 99.96, 90.00 |
Refinement procedure
| Resolution | 47.570 - 3.090 |
| R-factor | 0.2362 |
| Rwork | 0.233 |
| R-free | 0.26920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.656 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.570 | 3.200 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.109 | |
| Number of reflections | 35605 | 1958 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 94.1 | |
| Redundancy | 3 | |
| CC(1/2) | 0.980 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% w/v PEG 3350, 0.1 M Lithium Sulfate |
