8V1L
Crystal structure of the NTF2L domain of human G3BP1 in complex with small molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-22 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9655 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.170, 84.690, 102.150 |
| Unit cell angles | 90.00, 91.26, 90.00 |
Refinement procedure
| Resolution | 46.870 - 2.680 |
| R-factor | 0.28101 |
| Rwork | 0.276 |
| R-free | 0.39465 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.179 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.870 | 2.749 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.337 | |
| Number of reflections | 23085 | 1685 |
| <I/σ(I)> | 0.281 | |
| Completeness [%] | 96.5 | 95.91 |
| Redundancy | 1.05 | |
| CC(1/2) | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M MES pH 6.5, 15% PEG 550 MME |
