8UWZ
The structure of Raamsizumab in complex with VEGF121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 31 |
| Unit cell lengths | 167.747, 167.747, 42.501 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 83.870 - 3.500 |
| R-factor | 0.2433 |
| Rwork | 0.237 |
| R-free | 0.29810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.948 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.870 | 3.670 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.122 | 1.212 |
| Rmeas | 0.130 | 1.297 |
| Rpim | 0.046 | 0.458 |
| Number of reflections | 18391 | 3833 |
| <I/σ(I)> | 9.2 | 1.6 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.7 | 7.8 |
| CC(1/2) | 0.999 | 0.769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 298 | 0.2 M Sodium Phosphate mono basic, 30% PEG600 |
