8UTM
CryoEM Structure of Allosterically Switchable De Novo Protein sr322, In Closed State without Effector Peptide, off Target Multimeric State
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.32 |
| Number of Particle | 699357 |
| Software | Coot,PyMOL,PHENIX,cryoSPARC,UCSF ChimeraX,ISOLDE |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
