8URI

Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 27 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome

This is a non-PDB format compatible entry.

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	FEI TALOS ARCTICA
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	5.3
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION