8URF
Crystal Structure of human ASGR2 CRD (Carbohydrate Recognition Domain) bound to 8G8 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 102.410, 102.410, 358.985 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.544 - 1.900 |
| R-factor | 0.1679 |
| Rwork | 0.166 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.225 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14rc1_3177: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.107 | 3.193 |
| Rmeas | 0.109 | 3.273 |
| Rpim | 0.025 | 0.718 |
| Number of reflections | 57655 | 3681 |
| <I/σ(I)> | 16.3 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.8 | 20.7 |
| CC(1/2) | 0.999 | 0.440 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 100mM sodium HEPES pH 7.5, 100mM calcium chloride, 30% (v/v) PEG400 Cryo prodtecion with 16% (v/v) glycerol |
