8UQR
Crystal structure of the human p53 tetramerization domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE 7B2 |
Synchrotron site | CHESS |
Beamline | 7B2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.973, 56.437, 61.656 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.630 - 1.220 |
R-factor | 0.1461 |
Rwork | 0.144 |
R-free | 0.17670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 1.763 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.630 | 1.264 |
High resolution limit [Å] | 1.220 | 1.220 |
Rmerge | 0.045 | |
Number of reflections | 30355 | 1953 |
<I/σ(I)> | 19.68 | |
Completeness [%] | 91.8 | |
Redundancy | 5.5 | |
CC(1/2) | 1.000 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 15% PEG 1500 |