8UQE

Crystal structure of RNF168 (RING)-UbcH5c fused to H2A-H2B via a 26-residue linker (RING not modeled in density)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	109.279, 109.279, 86.782
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.540 - 3.562
R-factor	0.251
Rwork	0.249
R-free	0.26760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5egg
RMSD bond length	0.003
RMSD bond angle	0.501
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.620
High resolution limit [Å]	3.562	3.562
Rmerge	0.118	0.705
Rmeas	0.128	0.761
Rpim	0.048	0.281
Number of reflections	7433	720
<I/σ(I)>	13	2.46
Completeness [%]	99.3	98.61
Redundancy	6.8	6.7
CC(1/2)	0.999	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	Crystals were obtained by mixing 2 microliters of the protein fusion (10 mg/mL) in 10 mM HEPES, pH 7.5, 600 mM NaCl, 1 mM TCEP and 2 microliters of the reservoir solution containing 0.1 M imidazole, pH 6.5, 1.0 M sodium acetate