8UQB

Crystal structure of RNF168 (RING)-UbcH5c fused to H2A-H2B via a 20-residue linker (crystallization condition 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	101.948, 101.948, 86.992
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.370 - 2.484
R-factor	0.2223
Rwork	0.218
R-free	0.25960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gb0 5egg
RMSD bond length	0.004
RMSD bond angle	0.656
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.573
High resolution limit [Å]	2.484	2.484
Rmerge	0.113	0.777
Rmeas	0.126	0.877
Rpim	0.053	0.396
Number of reflections	18662	1718
<I/σ(I)>	16.39	2.66
Completeness [%]	99.4	94.24
Redundancy	5.3	4.6
CC(1/2)	0.996	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	288	Crystals were obtained by mixing 2 microliters of the protein fusion (12 mg/mL) in 10 mM HEPES, pH 7.5, 600 mM NaCl, 1 mM TCEP and 2 microliters of the reservoir solution containing 50 mM BIS-TRIS propane, pH 7.0, 1 M NaCl