8UQA

Crystal structure of RNF168 (RING)-UbcH5c fused to H2A-H2B via a 12-residue linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	102.953, 102.953, 87.231
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.179 - 2.049
R-factor	0.1943
Rwork	0.192
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gb0 5egg
RMSD bond length	0.003
RMSD bond angle	0.554
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.122
High resolution limit [Å]	2.049	2.049
Rmerge	0.099	0.833
Rmeas	0.102	0.869
Rpim	0.027	0.243
Number of reflections	33909	3326
<I/σ(I)>	31.56	4.73
Completeness [%]	99.8	99.52
Redundancy	14.2	12.6
CC(1/2)	0.999	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	Crystals were obtained by mixing 2 microliters of the protein fusion (8.0 mg/mL) in 10 mM HEPES, pH 7.5, 600 mM NaCl, 1 mM TCEP and 2 microliters of the reservoir solution containing 0.1 M HEPES, pH 7.5, 2.4 M NaCl