8UMZ
Crystal Structure of Engineered Mouse Protocadherin-15 EC4-EC7 Connection
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.276, 50.613, 88.263 |
| Unit cell angles | 90.00, 94.01, 90.00 |
Refinement procedure
| Resolution | 49.200 - 1.630 |
| R-factor | 0.22276 |
| Rwork | 0.223 |
| R-free | 0.27354 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.703 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.630 | 1.640 |
| Rmerge | 0.074 | 0.406 |
| Rmeas | 0.091 | |
| Rpim | 0.052 | 0.277 |
| Number of reflections | 54156 | 2557 |
| <I/σ(I)> | 10.956 | 2.293 |
| Completeness [%] | 93.6 | 96.2 |
| Redundancy | 2.8 | 2.9 |
| CC(1/2) | 0.997 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M Sodium cacodylate pH 6.5,40% v/v MPD,5% w/v PEG 8000 |
