8ULJ
Prefusion RSV F bound by neutralizing antibody 2E08
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 164.715, 164.715, 146.157 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.390 - 3.000 |
| R-factor | 0.2382 |
| Rwork | 0.237 |
| R-free | 0.26350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 5.240 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.390 | 3.108 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 45920 | 4550 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 98.4 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.980 | 0.952 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 23.64%w/v PEG 1500, 0.1 M MMT buffer (DL-malic acid:MES:Tris base at a molar ratio of 1:2:2) with pH 4.36 |
