8UKX

Crystal structure the extracellular region of the epidermal growth factor receptor variant III (EGFRvIII) at pH 7.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-11
Detector	MAR CCD 300 mm
Wavelength(s)	1.0722
Spacegroup name	P 21 21 21
Unit cell lengths	43.579, 97.127, 115.323
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.580 - 3.301
R-factor	0.2878
Rwork	0.286
R-free	0.31970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.020
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (14-JUN-2023))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.580	3.480
High resolution limit [Å]	3.300	3.300
Rmerge	0.073	0.733
Rmeas	0.084	0.841
Rpim	0.041	0.403
Total number of observations	30394	4405
Number of reflections	7669	1089
<I/σ(I)>	10.9	1.4
Completeness [%]	98.2
Redundancy	4	4
CC(1/2)	0.999	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	7mg/ml protein, 27.5% PEG 3350, 5% glycerol, 100 mM HEPES, pH 7.0, +5% glycerol for cryo