8UK5
Crystal structure of the bromodomain of human ATAD2B in complex with histone H4S1(ph)K5ac
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2020-07-12 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.080, 48.580, 83.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.370 - 1.400 |
Rwork | 0.178 |
R-free | 0.20230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.865 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.370 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 33447 | 37316 |
<I/σ(I)> | 28.93 | |
Completeness [%] | 99.5 | |
Redundancy | 11.9 | |
CC(1/2) | 1.000 | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.2 M ammonium sulfate, 0.1 M BIS-TRIS (pH 5.5), 25% w/v PEG 3350 |