8UGO

Structure of the complex between Human LIAS and H-protein in the presence of 5'-deoxyadenosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2021-09-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	48.681, 169.209, 183.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.060 - 2.450
R-factor	0.1862
Rwork	0.185
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.584
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.530
High resolution limit [Å]	2.450	2.450
Number of reflections	320427	4299
<I/σ(I)>	7.78
Completeness [%]	94.5
Redundancy	3.3
CC(1/2)	0.976	0.348

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Potassium phosphate pH8.0, 0.2 M potassium chloride, 20% PEG 3350