8UFY

High Resolution structure of A. viridans Lactate Oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.7293
Spacegroup name	I 4
Unit cell lengths	132.262, 132.262, 91.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.700 - 1.200
R-factor	0.1467
Rwork	0.146
R-free	0.16190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.805
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5084)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.350	1.190
High resolution limit [Å]	1.170	1.170
Rpim	0.040	0.801
Number of reflections	517344	13078
<I/σ(I)>	9.7	1.1
Completeness [%]	99.9	100
Redundancy	4.7
CC(1/2)	0.998	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	26-30.5% ETHYLENE GLYCOL, 100 mM MES ph 4.0, 125mM sodium Lactate