8UFK

Human PU.1 ETS-Domain (165-270) Bound to d(AATAAAAGCGGAAGTG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.920119
Spacegroup name	P 1 21 1
Unit cell lengths	55.459, 70.714, 58.879
Unit cell angles	90.00, 114.00, 90.00

Refinement procedure

Resolution	31.100 - 2.410
R-factor	0.2315
Rwork	0.228
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.642
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.100	2.500
High resolution limit [Å]	2.410	2.410
Rmerge	0.076	0.625
Rmeas	0.084	0.693
Rpim	0.036	0.295
Number of reflections	16100	1678
<I/σ(I)>	9.5	2
Completeness [%]	99.6	99
Redundancy	5.3	5.1
CC(1/2)	0.994	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	100mM TRIS Base (HCl adjusted), pH=8.5, 200mM Ammonium Acetate, 24% PEG3350 1:1 hanging drop with 250uM protein/DNA complex