8UDV
The X-RAY co-crystal structure of human FGFR3 V555M and Compound 17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.664, 54.513, 128.718 |
| Unit cell angles | 90.00, 120.22, 90.00 |
Refinement procedure
| Resolution | 34.740 - 2.348 |
| R-factor | 0.2374 |
| Rwork | 0.236 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.010 |
| Data reduction software | autoPROC (1.1.7) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.740 | 2.430 |
| High resolution limit [Å] | 2.348 | 2.348 |
| Rmerge | 0.090 | 0.640 |
| Rmeas | 0.120 | 0.810 |
| Rpim | 0.070 | 0.490 |
| Number of reflections | 42440 | 4114 |
| <I/σ(I)> | 8.8 | 2.3 |
| Completeness [%] | 98.3 | 96.2 |
| Redundancy | 2.8 | 2.6 |
| CC(1/2) | 0.990 | 0.470 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.10 M BIS-TRIS/HCl pH 6.50, 0.20 M (NH4)2SO4, 25.00 % (w/v) PEG 3350 |
