8UD6

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-13
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	210.000, 449.390, 623.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	182.260 - 2.700
R-factor	0.2032
Rwork	0.201
R-free	0.24150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.808
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	182.260	182.260	2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.149	0.044	1.566
Rmeas	0.170	0.050	1.777
Total number of observations	6912983
Number of reflections	1582645	18098	116704
<I/σ(I)>	8.02	26.37	0.94
Completeness [%]	99.4	96.1	99.5
Redundancy	4.368	4.464	4.404
CC(1/2)	0.992	0.996	0.227

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME