8UCT
Crystal structure of TcPINK1 in complex with PRT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-27 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 53.216, 53.216, 545.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 90.930 - 2.930 |
| R-factor | 0.2845 |
| Rwork | 0.283 |
| R-free | 0.30520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.776 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.930 | 2.980 |
| High resolution limit [Å] | 2.930 | 2.930 |
| Rpim | 0.145 | |
| Number of reflections | 11014 | 521 |
| <I/σ(I)> | 6.1 | 0.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.8 | 16.9 |
| CC(1/2) | 0.995 | 0.551 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 100mM HEPES, PEG 4000, 150mM ammonium sulfate |
