8U7M
Human retinal variant phosphomimetic IMPDH1(595)-S477D free octamer bound by GTP, ATP, IMP, and NAD+
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.1 |
| Number of Particle | 343619 |
| Software | cryoSPARC,PHENIX,Leginon,UCSF ChimeraX,ISOLDE,Coot |
| Symmetry | D4 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
