8U7J
Crystal Structure of Staphylococcus aureus PLP synthase complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2021-08-15 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 |
| Unit cell lengths | 101.235, 132.374, 142.097 |
| Unit cell angles | 106.49, 109.51, 105.56 |
Refinement procedure
| Resolution | 54.830 - 3.020 |
| R-factor | 0.2022 |
| Rwork | 0.200 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.454 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_4788: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.239 | 3.389 |
| High resolution limit [Å] | 3.019 | 3.019 |
| Rmerge | 0.127 | 0.561 |
| Rpim | 0.075 | 0.329 |
| Number of reflections | 71124 | 3556 |
| <I/σ(I)> | 4.7 | 1.6 |
| Completeness [%] | 59.3 | 10.1 |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.993 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 5% PEG4000, 0.2 M triammonium citrate pH 7, 10 mM L-glutamine |
