8U70

Crystal structure of malaria transmission-blocking anti-Pfs48/45 antibody RUPA-58.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-06-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	P 32
Unit cell lengths	164.513, 164.513, 92.276
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.460 - 3.090
R-factor	0.2245
Rwork	0.223
R-free	0.26310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.907
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.460	3.200
High resolution limit [Å]	3.090	3.090
Rpim	0.152	0.517
Number of reflections	51007	5024
<I/σ(I)>	3.6	1.4
Completeness [%]	99.7	99
Redundancy	9.5	9
CC(1/2)	0.957	0.336

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M sodium acetate, 30% PEG 8000, 0.1 M sodium cacodylate, 20% ethylene glycol