8TX4

Crystal Structure of rA3G-ssDNA-GA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2022-12-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	P 21 21 21
Unit cell lengths	55.295, 67.977, 127.906
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.950 - 1.900
R-factor	0.1767
Rwork	0.175
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.822
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.950	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.106	0.978
Rpim	0.030	0.281
Number of reflections	38683	3806
<I/σ(I)>	17.48	1.61
Completeness [%]	99.7	99.71
Redundancy	13.1	12.6
CC(1/2)	0.999	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M Bis-tris propane pH 7.5, 0.2 M Sodium/Potassium phosphate, 16% PEG3350