8TW3

hMPV fusion protein complexed with single domain antibodies sdHMPV16 and sdHMPV12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	80.260, 115.610, 158.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.970 - 2.900
R-factor	0.1927
Rwork	0.191
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.501
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.970	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.214	0.862
Rmeas	0.237	0.951
Rpim	0.099	0.393
Total number of observations	181517	27228
Number of reflections	32688	4780
<I/σ(I)>	7	2.4
Completeness [%]	98.6
Redundancy	5.6	5.7
CC(1/2)	0.962	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	0.1M HEPES and 18% PEG 12,000