8TW3
hMPV fusion protein complexed with single domain antibodies sdHMPV16 and sdHMPV12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.260, 115.610, 158.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.970 - 2.900 |
R-factor | 0.1927 |
Rwork | 0.191 |
R-free | 0.23240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.501 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.970 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.214 | 0.862 |
Rmeas | 0.237 | 0.951 |
Rpim | 0.099 | 0.393 |
Total number of observations | 181517 | 27228 |
Number of reflections | 32688 | 4780 |
<I/σ(I)> | 7 | 2.4 |
Completeness [%] | 98.6 | |
Redundancy | 5.6 | 5.7 |
CC(1/2) | 0.962 | 0.638 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.1M HEPES and 18% PEG 12,000 |