8TVT
Structure of human Cysteine desulfurase Nfs1 with L-propargylglycine bound to active site PLP in complex with ISD11, Acp1 and ISCU2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9796 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.270, 86.270, 246.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.950 - 2.000 |
R-factor | 0.1519 |
Rwork | 0.150 |
R-free | 0.18630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.632 |
Data reduction software | AutoProcess |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.950 | 2.072 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.138 | 0.852 |
Number of reflections | 63786 | 6269 |
<I/σ(I)> | 12.47 | |
Completeness [%] | 100.0 | |
Redundancy | 7.9 | |
CC(1/2) | 0.997 | 0.818 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288 | 0.1 M MES pH 6.5 22.5 % PEG 400 |