8TV1

Structure of the EphA2 LBDCRD bound to FabS1C_L1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	123.437, 73.249, 118.570
Unit cell angles	90.00, 107.43, 90.00

Refinement procedure

Resolution	62.200 - 2.600
R-factor	0.2048
Rwork	0.202
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.040
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.200	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.130	1.102
Rmeas	0.149	1.269
Rpim	0.072	0.619
Number of reflections	28513	3018
<I/σ(I)>	6.2	1.7
Completeness [%]	91.4	79.4
Redundancy	3.9	3.8
CC(1/2)	0.989	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	14% PEG 20K, 100 mm HEPES 7.0, 200 mM KSCN