8TUU

MW Polyomavirus LTA bipartite NLS bound to importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	77.390, 90.067, 100.146
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.350 - 2.500
R-factor	0.2037
Rwork	0.202
R-free	0.23530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.513
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.350	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.120	1.090
Rmeas	0.130	1.180
Rpim	0.050	0.450
Number of reflections	24858	2728
<I/σ(I)>	11.9	2
Completeness [%]	99.9
Redundancy	6.7
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.7 M sodium citrate, 0.1 M HEPES pH 7.5, 0.01 M DTT