8TUU
MW Polyomavirus LTA bipartite NLS bound to importin alpha 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-09 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.390, 90.067, 100.146 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.350 - 2.500 |
R-factor | 0.2037 |
Rwork | 0.202 |
R-free | 0.23530 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.513 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.350 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.120 | 1.090 |
Rmeas | 0.130 | 1.180 |
Rpim | 0.050 | 0.450 |
Number of reflections | 24858 | 2728 |
<I/σ(I)> | 11.9 | 2 |
Completeness [%] | 99.9 | |
Redundancy | 6.7 | |
CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.7 M sodium citrate, 0.1 M HEPES pH 7.5, 0.01 M DTT |