8TTM
IgG1 Fc Heterodimer combYSelect1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.782, 79.394, 142.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.880 - 2.510 |
R-factor | 0.20601 |
Rwork | 0.202 |
R-free | 0.24250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.446 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PDB-REDO |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.880 | 2.600 |
High resolution limit [Å] | 2.510 | 2.510 |
Rmerge | 0.240 | 0.961 |
Rmeas | 0.254 | 1.020 |
Rpim | 0.079 | 0.336 |
Number of reflections | 19700 | 1814 |
<I/σ(I)> | 30.3 | 2.25 |
Completeness [%] | 98.1 | |
Redundancy | 10.2 | |
CC(1/2) | 0.993 | 0.852 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.1M BisTris pH 6.5 and 21% PEG MME 5000 |