8TS7

Human PI3K p85alpha/p110alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	87.990, 120.330, 190.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.790 - 2.710
R-factor	0.2603
Rwork	0.259
R-free	0.28840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.460
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.790	2.780
High resolution limit [Å]	2.710	2.710
Rmerge	0.076
Rmeas	0.081
Rpim	0.030
Number of reflections	55887	4066
<I/σ(I)>	12.8
Completeness [%]	100.0	99.9
Redundancy	7.4	7.3
CC(1/2)	0.999	0.321

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M Hepes pH 7.5, 9% PEG-3350