8TQ8
Crystal structure of Fab.34.5.8 in complex with MHC-I (H2-Dd)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 273 |
Detector technology | PIXEL |
Collection date | 2022-09-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 87.087, 50.892, 224.825 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.090 - 2.690 |
R-factor | 0.2085 |
Rwork | 0.201 |
R-free | 0.24250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5weu |
RMSD bond length | 0.004 |
RMSD bond angle | 0.756 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.090 | 2.800 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.145 | 0.927 |
Rpim | 0.085 | 0.455 |
Number of reflections | 54120 | 5458 |
<I/σ(I)> | 7.6 | 1.3 |
Completeness [%] | 97.9 | 99.3 |
Redundancy | 3.8 | |
CC(1/2) | 0.990 | 0.565 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 18% PEG4000, 0.1 M MES, pH 6.0, 0.12 M calcium acetate |