8TOO

Crystal structure of Epstein-Barr virus gp42 in complex with antibody 4C12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	53.296, 63.568, 199.278
Unit cell angles	89.81, 89.75, 88.86

Refinement procedure

Resolution	53.510 - 2.600
R-factor	0.2281
Rwork	0.226
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.816
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.420	2.600
High resolution limit [Å]	2.600	2.600
Number of reflections	73888	7047
<I/σ(I)>	5.4
Completeness [%]	91.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20mM Tris pH7.5, 150mM NaCl, 0.2M zinc acetate dihydrate, and 18% PEG 3,350