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8TOE

Escherichia coli RNA polymerase unwinding intermediate (I1c) at the lambda PR promoter

EM specimen
VitrificationYes
InstrumentSPOTITON
EM Data Collection
MicroscopeFEI TITAN KRIOS
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
Energy FilterGIF Bioquantum
DetectorGATAN K3 (6k x 4k)
3D Reconstruction
MethodSINGLE PARTICLE
Resolution (Å)2.9
SoftwarecryoSPARC,Leginon
CTF correctionPHASE FLIPPING AND AMPLITUDE CORRECTION

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PDB entries from 2024-11-20

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