8TOE
Escherichia coli RNA polymerase unwinding intermediate (I1c) at the lambda PR promoter
EM specimen
Vitrification | Yes |
Instrument | SPOTITON |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.9 |
Software | cryoSPARC,Leginon |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |