8TM2
Preclinical Characterization of Pan-NKG2D Ligand-Binding NKG2D Receptor Decoys
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.571, 69.782, 145.374 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.690 - 2.850 |
R-factor | 0.21573 |
Rwork | 0.214 |
R-free | 0.25284 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.141 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 72.690 | 50.000 | 2.900 |
High resolution limit [Å] | 2.850 | 7.730 | 2.850 |
Rmerge | 0.092 | 0.045 | 0.502 |
Rmeas | 0.056 | 0.615 | |
Rpim | 0.033 | 0.349 | |
Number of reflections | 11703 | 608 | 613 |
<I/σ(I)> | 10.2 | ||
Completeness [%] | 83.2 | 76.4 | 87.2 |
Redundancy | 2.7 | 2.4 | 2.7 |
CC(1/2) | 0.996 | 0.794 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Magnesium Chloride, Tris (pH 8.5), PEG3350 |