8TK6

HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE (VHR) in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-16
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.97949
Spacegroup name	P 1
Unit cell lengths	34.896, 41.283, 59.118
Unit cell angles	86.58, 89.67, 75.06

Refinement procedure

Resolution	59.010 - 1.650
R-factor	0.14773
Rwork	0.146
R-free	0.18336
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.581
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.010	1.730
High resolution limit [Å]	1.650	1.650
Rmerge	0.037	0.078
Number of reflections	32373	2000
<I/σ(I)>	12.8	9.85
Completeness [%]	88.7	51
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	Protein in 50 mM TRIS-Cl pH 8.5, 1 mM TCEP, 0.5 mM EDTA was mixed with the precipitant solution (100 mM Bis-Tris pH 6.5, 50 mM NH4F, 28% (w/v) PEG-4000) and equilibrated against the precipitant solution.