8TK4
HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE (VHR) complexed with phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.086, 54.703, 100.833 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.100 - 1.800 |
R-factor | 0.15628 |
Rwork | 0.155 |
R-free | 0.19098 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.539 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.100 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.101 | 0.791 |
Number of reflections | 17592 | 1027 |
<I/σ(I)> | 13.6 | 3.1 |
Completeness [%] | 96.9 | 98 |
Redundancy | 6.7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | protein in 50 mM Tris-Cl, pH 8.0, 1 mM TCEP was mixed with the precipitation buffer (0.1M Sodium potassium phosphate pH 7.0, 28% w/v Polyethylene glycol 3,350) and equilibrated against it |