8TJT
The Fab fragment of an anti-glucagon receptor (GCGR) antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0005 |
Spacegroup name | H 3 |
Unit cell lengths | 203.013, 203.013, 137.285 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.780 - 2.700 |
R-factor | 0.2057 |
Rwork | 0.204 |
R-free | 0.25170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.466 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.20rc3-4406_final) |
Refinement software | PHENIX (1.20rc3-4406_final) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.780 | 2.797 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.088 | |
Rmeas | 0.098 | |
Rpim | 0.043 | |
Number of reflections | 57903 | 5827 |
<I/σ(I)> | 17.81 | 2.03 |
Completeness [%] | 99.9 | 99.97 |
Redundancy | 5.2 | 5 |
CC(1/2) | 0.998 | 0.705 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium citrate, 20 % (v/v) polypropylene glycol 3350, 0.1 M lithium chloride |