8TJF
monovalent bispecific IgG antibodies through novel electrostatic steering mutations at the CH1-CL interface
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9741 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.783, 78.698, 75.658 |
Unit cell angles | 90.00, 91.84, 90.00 |
Refinement procedure
Resolution | 37.838 - 2.300 |
Rwork | 0.208 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.556 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.350 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.127 | |
Number of reflections | 48672 | 3825 |
<I/σ(I)> | 11.1 | |
Completeness [%] | 99.0 | |
Redundancy | 6.9 | |
CC(1/2) | 0.998 | 0.678 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 200 mM sodium chloride, 0.1 M Bis-Tris at pH 5.5, 25% w/v PEG 3350 |