8TJA

CRYSTAL STRUCTURE OF THE A/Ecuador/1374/2016(H3N2) INFLUENZA VIRUS HEMAGGLUTININ WITH HUMAN RECEPTOR ANALOG 6'-SLNLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	H 3 2
Unit cell lengths	100.687, 100.687, 394.716
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.940 - 2.050
R-factor	0.1812
Rwork	0.180
R-free	0.20690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.543
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.940	2.140
High resolution limit [Å]	2.050	2.050
Rpim	0.040	0.300
Number of reflections	49313	6056
<I/σ(I)>	19.8	2.8
Completeness [%]	100.0
Redundancy	9.4
CC(1/2)	0.996	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	50% PEG-200, 0.05 M Lithium sulfate, and 0.1M Tris pH 7.0