8TJ4
CRYSTAL STRUCTURE OF THE A/Bangkok/1/1979(H3N2) INFLUENZA VIRUS HEMAGGLUTININ WITH HUMAN RECEPTOR ANALOG 6'-SLNLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | H 3 |
| Unit cell lengths | 100.655, 100.655, 687.903 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.230 - 1.950 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.22270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.957 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.230 | 2.030 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rpim | 0.070 | 0.470 |
| Number of reflections | 188791 | 21037 |
| <I/σ(I)> | 13.1 | 1.2 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 4.9 | 4.8 |
| CC(1/2) | 0.989 | 0.738 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 40% PEG-600 and 0.1 M CHES pH 9.5 |
