8TH5
Crystal Structure of the G3BP1 NTF2-like domain bound to the IDR1 of SARS-CoV-2 nucleocapsid protein P13L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.600, 69.450, 101.040 |
| Unit cell angles | 70.66, 76.86, 88.31 |
Refinement procedure
| Resolution | 46.700 - 2.620 |
| R-factor | 0.23099 |
| Rwork | 0.226 |
| R-free | 0.33622 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.217 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.770 | 2.740 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.370 | |
| Number of reflections | 31252 | 440 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 71.4 | 7.8 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.898 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277.15 | 0.2 M lithium sulfate, 0.1 M Tris pH 8.5, 25% PEG3350 |
