8TGO

Crystal structure of the BG505 triple tandem trimer gp140 HIV-1 Env in complex with PGT124 and 35O22

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	357.592, 212.044, 207.710
Unit cell angles	90.00, 125.07, 90.00

Refinement procedure

Resolution	41.080 - 5.750
R-factor	0.2441
Rwork	0.242
R-free	0.29160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cez
RMSD bond length	0.003
RMSD bond angle	0.587
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000	60.000	5.900
High resolution limit [Å]	5.750	15.650	5.800
Rmerge	0.221	0.074	1.763
Rmeas	0.239	0.081	1.941
Rpim	0.091	0.032	0.794
Number of reflections	33735	1589	1623
<I/σ(I)>	5.3
Completeness [%]	97.8	89	94
Redundancy	6.8	6	5.7
CC(1/2)	0.992	0.996	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.1 M sodium acetate, 0.2 M zinc acetate, 11.5 % w/v PEG3000, pH 4.83